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process for rapid in-line purification by desalting of proteins and oligopeptides

Epigem made a microfluidic device for a group at the University of Western Ontario to demonstrate the separation of salts and low molecular contaminants from biological polymer solutions using two laminar flow layers (D.J.Wilson, L. Konermann, Analytical Chemistry, Vol. 77, No. 21, November 1, 2005). The device was used for purification of proteins and brodykin (a nine amino acid oligopeptide) prior to electrospray mass spectral analysis (ESI-MS) – 90% of the contaminating salt was removed. The device may facilitate the rapid analysis of proteins and other macromolecules in biological matrixes, such as urine or blood, or in complex mixtures..